Pregnancy test, test the level of progesterone in the urine, not FSH and LH. FSH cause the follicle to develop with the help of granulosa cells, and theca cells. Once developed LH will cause the follicle to ovulate their for releasing the oocyte. High level of progesterone only tell you if the follicle has developed a CL. Therefore detecting pre-adrenal in ferrets through a pregnancy test wouldn't be sufficient. Being that the ferrets are spayed the constant stimulation of LHRH to the Anterior Pituitary to the gonadatrophs would secret constant levels of FSH/LH. There is no negative feedback from inhibin or testosterone to the hypothalamus to slow down stimulation for LH/FSH. This is a main reason why adrenal is so common in every ferret. Unless we can develop a way to keep the gonads but not allow ovulation to occur in females, and the sexual behavior in males. Would be funny to see! One question: Are ferrets induced ovulators? [Sure are! BIG] Andria Ludwig [Posted in FML issue 5194]